Embark on a journey beyond traditional immunization: unveiling the potential of genetic immunization for unparalleled affinity with anti-idiotypic antibodies (anti-IDs). Genetic immunization unlocks a vast pool of highly diverse, high-affinity antibodies beyond conventional protein immunization. Our approach combines cutting-edge immunization strategies, multiple hybridoma and single B cell screening platforms, and Gator Bio's biolayer interferometry to generate and characterize best-in-class anti-IDs. This presentation illustrates that these technologies efficiently identify a robust panel of high-affinity anti-IDs that are ideal for pharmacokinetic applications.