Characterization and Quantification of Oligonucleotide and RNA Impurities by CE and LC-MS

Stephen Lock, PhD, Senior Market Development Manager, SCIEX

Characterization and Quantification of Oligonucleotide and RNA Impurities by CE and LC-MS

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In this presentation learn how a QTOF system can be used for high resolution quantitation of oligonucleotides providing the performance of a triple quadrupole. Learn how the improvements in sensitivity in MS/MS scanning and quantitation together with new software solutions are enabling users to easily identify impurities and improve the characterization of oligonucleotides and understand how LCMSMS can be used with ion pairing or HILIC chromatography to detect oligonucleotides in biological extracts.

Dr. Stephen Lock obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. Previously a lecturer of Chemistry at the University of Hull he has worked for SCIEX™ in various technical roles for over 25 years. He has over 30 years’ experience in Analytical Chemistry and has presented at nearly one hundred international meetings around the world. Steve is currently the EMEAI BioPharma Senior Market Development Manager for SCIEX and is also an Adjunct Professor at the University of Pannonia where he acts in a volunteer role as a Scientific Advisor for the Horváth Csaba Memorial Laboratory lead by Prof. Andras Guttman. At the Horváth Csaba Memorial Laboratory Steve provides technical advice and supports the groups fundamental research and development of new applications for capillary electrophoresis.