Strategies for Oligonucleotide Purification Using Reverse Phase Resins

Martin Deetz, PhD, Senior Technical Laureate at DuPont

Strategies for Oligonucleotide Purification Using Reverse Phase Resins

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Oligonucleotides are short synthetic DNA sequences that are used for diagnostic and therapeutic purposes. They are manufactured through multi-step chemical synthesis processes which are prone to generate impurities. Multiple options for oligonucleotide purification are available....but which route is best? In this presentation we explore oligonucleotide purification challenges in process development and production through different purification examples. We will discuss: 1) Comparison of reverse phase and ion exchange purification strategies; 2) Reverse phase chromatography options for DMT-On and DMT-Off purification and 3) Purification of short and long oligonucleotides.

Dr. Martin Deetz is a Senior Technical Laureate at DuPont, focusing on specialty separations and new product developments in bioprocessing. In his previous role, he was the global R&D leader for Ion Exchange Resins and Specialty Membranes overseeing the development of new products across all water and specialty market segments. Martin has worked with ion exchange resins and membrane products (RO / NF / UF) for over 20 years in roles within the businesses of DuPont, Dow and Rohm and Haas.