Two Enzymatic Approaches for Large-scale siRNA Synthesis

Matthew Miller PhD, Director, Live Science and RNA Technology, Codexis

Two Enzymatic Approaches for Large-scale siRNA Synthesis

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In this presentation, we will discuss two enzymatic approaches for siRNA synthesis that are commercially relevant to address the foreseeable market demands. First, a ligation method utilizing engineered dsRNA ligases to enable assembly of crude shortmer oligos synthesized by SPOS. Second, a polymerase-based oligonucleotide synthesis technology for the linear, stepwise manufacturing of siRNA therapeutics assets.

Dr. Miller joined Codexis in 2009, and he was instrumental in several enzyme engineering projects including T7 RNA polymerase evolution for mRNA manufacturing and TdT evolution for enzymatic DNA synthesis. He is currently focused on enzymes for siRNA synthesis. Dr. Miller received his Ph.D. from the University of California, San Francisco.