LC-MS Applications for Characterization of Short Synthetic Oligonucleotides, sgRNAs and mRNAs

Catalin Doneanu, PhD, Principal Chemist, Application Science & Evaluation Team, Waters Corporation

LC-MS Applications for Characterization of Short Synthetic Oligonucleotides, sgRNAs and mRNAs

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This presentation is focused on two recently launched software tools (INTACT Mass and CONFIRM Sequence) for LC-MS analysis of oligonucleotides. We will demonstrate how we can combine these two software Apps into an automated, compliance-ready LC-MS workflow for purity analysis, intact mass confirmation and sequence confirmation of short synthetic oligonucleotides (20-25-mers) and their impurities. In addition, several applications of the newly introduced wide pore (300Å) Premier BEH columns will be highlighted for separation of long-mer oligonucleotides (>40-mers) including single guide RNAs (sgRNAs), heterogeneous Poly(A) Tails as well as mRNA digests.

Catalin Doneanu, PhD, Principal Chemist, Application Science & Evaluation Team, Waters Corporation
Catalin Doneanu received his Ph.D. in analytical chemistry from Oregon State University in 2002. After a postdoctoral stage in the Department of Medicinal Chemistry at University of Washington in Seattle, he joined Waters Corporation in 2006. He is currently a Principal Chemist in the Biopharmaceutical Sciences group. Catalin has more than twenty years of experience in separation sciences and mass spectrometry. His areas of expertise in LC-MS include analysis of oligonucleotides, host cell protein analysis, MS based quantification of peptides and proteins, protein characterization and analysis or protein aggregates. Catalin has over 30 peer-reviewed publications.